sjs b7e30Sven Saupe, Institute of Cellular Biochemistry and Genetics, UMR 5095


I am CNRS research director, geneticist at the IBGC (Institute of Biochemistry and Cellular Genetics UMR 5095) and head of the "Non-Self Recognition in Fungi" team. We are interested in the mechanisms governing the recognition of non-self in this vast branch of the eukaryotic tree. We study various aspects of the detection and response to noself in fungi, with particular emphasis on a family of cytoplasmic receptors called NLRs (Nod-like receptors) that control innate immunity and other processes of biotic interaction in animal and plant lines. NLR proteins are intracellular receptors that constitute a conserved component of the innate immune system of cellular organisms. In fungi, NLRs are characterized by a wide diversity of architectures and the presence of amyloid signaling.

Amyloids are implicated in many diseases due to protein misfolding (Alzheimer's and Parkinson's diseases and type 2 diabetes). Functional amyloids involved in the control of cell death regulatory mechanisms have so far only been characterized in fungi and mammals. The Infranalytics project aimed to characterize a functional amyloid involved in NLR activation in bacteria. A method of choice for studying amyloids at the atomic level is solid-state nuclear magnetic resonance (NMR). The development of isotopic labeling strategies has greatly enhanced the power of solid-state NMR in structural studies, as the labeling patterns allow selective retrieval of desired information (i.e. specific position of labeled nuclei 13This 15N in amyloid proteins). This project was developed in collaboration with Antoine Loquet.

We have identified a number of bacterial amyloid signaling motifs in bacterial genomes that we call “tem BASS” (bacterial amyloid signaling sequence). During this Infranalytics project, we used solid-state NMR to assign the rigid core of the BASS1 protein to its fibrillar state.

 publi Saupe 1af68

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Platform used: CBMN - IECB (Bordeaux)