CBMN - IECB Bordeaux

Platform name
Institute of Chemistry & Biology of Membranes and Nano-objects - European Institute of Chemistry and Biology
City
Bordeaux
Address

European Institute of Chemistry & Biology
2 Rue Robert Escarpit,
33607 Pessac

Scientific Officer
Dr. Antoine Locket (This e-mail address is protected against spambots. You need JavaScript enabled to view it.)
Operational manager
Mrs. Axelle Grelard (This e-mail address is protected against spambots. You need JavaScript enabled to view it.)
Communication correspondent
Mrs. Estelle MorvanThis e-mail address is protected against spambots. You need JavaScript enabled to view it.)
196 dsc1240 2021 01 28 31654 iecb infrastructure
The institute of Chemistry and Biology of Membranes and Nano-objects (CBMN) is a joint research unit of the CNRS (main INC, secondary INSB), the University of Bordeaux and the Institut National Polytechnique de Bordeaux. The CBMN institute is also associated with Bordeaux Science Agro. CBMN has about 200 people (50% permanent and 50% non-permanent). It is a multidisciplinary institute and operates at the interface between Chemistry, Biology and Physical by hosting 16 research groups gathered in four thematic poles: Chemical biology and Supramolecular chemistry (CBSC), Biomedical research, Fundamental and translational (BioFAP), Multiscale Biophysics (MSB), Nutraceutical, medical and food applications of biomolecules (NMFAB).
 
 

The European Institute of Chemistry and Biology (IECB) is an incubator for international research teams at the chemistry-biology interface, placed under the joint supervision of the CNRS, theInserm and University of Bordeaux.
Its mission is to bring together the most promising European chemists and biologists in a multidisciplinary environment, in order to develop high-level research programs in collaboration with public and private research centres. About fifteen research teams certified by an International Scientific Council, i.e. nearly 180 researchers, technicians and students, work on the site every day.
Their expertises revolve around 4 axis of research: bio-organic chemistry and chemobiology, molecular biophysics, structural biology, molecular and cellular biology.


iecbext

 

nmr 6

The Bordeaux team
Photo credits: Marc Grémillon
 

Equipment

800 MHz

Magnetic field
800MHz
Description
Liquid, Solid and Soft Matter Applications
Specificities
Console: Bruker Avance NEO, 4 channels, Workstation: Linux environment, Topspin 4.X software
Measurement probes

 

  • Cryoprobe 5mm TCI 1H-13C/15N/2H with Z gradient, (S/N =x), T=4-50 °C
  • Solid probe 4mm, CPMAS 1H/2H/31P
  • Solid probe 3.2mm, CPMAS 1H/13C/15N
  • Solid probe 0.7mm, CPMAS 1H/2H/13C/15N
  • CPMAS 4H/BB 1 mm broadband solid probe

 

 

Expertise and services
  • NMR experiments in liquid medium (all multidimensional experiments including weakly orienting medium)
  • Experiments in soft, membrane, colloidal or liquid crystal media. Broad band, magic angle rotation and oriented samples
  • MAS and fast MAS experiments for biosolid samples (aggregates, pellets, membrane proteins, cells, cell wall)
  • Provision of equipment with or without assistance
  • Services
Other equipment

The IECB and CBMN NMR platform, made up of 8 spectrometers allowing liquid or solid analysis, ISO9001 and NF X50-900 certified since 2017, offers expertise for analytical applications:

  • Field of health, biology and biochemistry:
    • Structural biology: nucleic acids, proteins, protein-ligand interactions
    • Neuroscience: amyloid, Alzheimer's disease, Parkinson's disease, multiple sclerosis, prion disease
    • Virology: HBV, HCV
    • Peptides and proteins in lipid membranes
    • Encapsulation of active ingredients, drug-cell interaction
    • Bacterial infections, biofilms
    • Studies of solid dispersions of lipophilic active ingredients
  • Environment:
    • Vine dieback study
    • Wine protection / treatment
    • Study of wood
  • Food:
    • Lipids in food and cosmetic matrices
    • Role of tannins in the sensory perception of wine
  • Design materials:
    • Supramolecular chemistry
    • Electrode materials for batteries

192 dsc1234 2021 01 28 31648 iecb infrastructurePart of the NMR platform (photo credit: Marc Grémillon)

 

700 MHz Bruker Avance NEO Liquid / Solid

Measurement probes:

  • TXI (1H, 13C, 15N, 2H), 5 mm, z-gradients BBO (109Ag – 31P, 1H, 2H), 5 mm, z-gradients
  • HRMAS (1H, 13C, 15N, 2H), 4mm
  • MAS (1H, 2H, 31P), 4mm, E-free
  • MAS (15N – 31P, 1H), 4mm

600 MHz Bruker Advance NEO Solid State

Measurement probes:

  • MAS (1H,13C,15N), 4mm, E-free

  • MAS (1H,13C,15N), 3.2mm, E-free

  • MAS (1H, 13C, 15N, 2H), 1.3mm

  • MAS (1H, X), 4mm

500 MHz Bruker Advance III Solid

Measurement probes:

  • MAS (1H, X), 4mm

  • MAS (1H, 19F, X), 4mm

  • MAS (1H, X), 1.3mm

  • MAS (1H, X), 2,5mm

400 MHz Bruker Avance III HD Liquid

Measurement probe:

  • SmartProbe (15N-31P, 19F, 1H, 2H), 5mm z-gradients

Sample changer: Sample Case

300 MHz Bruker Advance III Solid

Measurement probes:

  • MAS (1H, X), 4mm

  • MAS (1H, X, Y), 4 mm, high temperatures (< 300°C)

  • MAS (1H, X), 2.5mm

300 MHz Bruker Advance II Liquid

Measurement probe:

  • BBFO (15N-31P, 1H, 2H), 5mm, z-gradients

100 MHz Bruker Advance II Solid

Measurement probe:

  • CPMAS (X, Y), 4mm

Publications

1 - Laurent Latxague, Sebastien Benizri, Alexandra Gaubert, James Tolchard, Denis Martinez, Estelle Morvan, Axelle Grelard, Ahmad Saad, Birgit Habenstein, Antoine Laquet, Philippe Barthélémy, Bolaamphiphile-based supramolecular gels with drugs eliciting membrane effects, Journal of Colloid and Interface Science, Volume 594, 2021, Pages 857-863, ISSN 0021-9797, https://doi.org/10.1016/j.jcis.2021.03.026

2 - Daskalov A, El Mammeri N, Lends A, Shenoy J, Lamon G, Fichou Y, Saad A, Martinez D, Morvan E, Berbon M, Grelard A, Kauffmann B, Ferber M, Bardiaux B, Habenstein B, Saupe SJ, Latch A. Structures of Pathological and Functional Amyloids and Prions, a Solid-State NMR Perspective. Front Mol Neurosci. 2021 Jul 1;14:670513, https://doi.org/10.3389/fnmol.2021.670513

3 - Haidar, R., Yacoub, A., Vallance, J., Compant, S., Antonielli, L., Saad, A., Habenstein, B., Kauffmann, B., Grelard, A., Laquet, A., Attard, E., Guyoneaud, R. and Rey, P. (2021), Bacteria associated with wood tissues of Esca-diseased grapevines: functional diversity and synergy with Fomitiporia mediterranea to degrade wood components. Environ Microbiol, 23:6104-6121. https://doi-org.inc.bib.cnrs.fr/10.1111/1462-2920.15676

4 - Structural and molecular basis of cross-seeding barriers in amyloids, Asen Daskalov, Denis Martinez, Virginie Coustou, Nadia El Mammeri, Mélanie Berbon, Loren B. Andreas, Benjamin Bardiaux, Jan Stanek, Abdelmajid Noubhani, Brice Kauffmann, Joseph S. Wall, Guido Pintacuda, Sven J. Saupe, Birgit Habenstein, Antoine Laquet, Proceedings of the National Academy of Sciences Jan 2021, 118 (1) e2014085118; DOI: 10.1073/pnas.2014085118

5 - The beginning and the end: flanking nucleotides induce a parallel G-quadruplex topology, Jielin Chen, Mingpan Cheng, Gilmar F Salgado, Petr Stadlbauer, Xiaobo Zhang, Samir Amrane, Aurore Guédin, Fangni He, Jiří Šponer, Huangxian Ju, Jean -Louis Mergny, Jun Zhou, Nucleic Acids Research 49 (16), 9548-9559. https://doi.org/10.1093/nar/gkab681

6 - Collie GW, Lombardo CM, Yo SH, Pulka-Ziach K, Gabelica V, Mackereth CD, Rosu F, Guichard G. “Crystal structures capture multiple stoichiometric states of an aqueous self-assembling oligourea foldamer”, Chem. Common. (2021), 10.1039/D1CC03604A.

7 - Lends, A., Berbon, M., Habenstein, B. et al. Protein resonance assignment by solid-state NMR based on 1H-detected 13C double-quantum spectroscopy at fast MAS. J Biomol NMR (2021), https://doi-org/10.1007/s10858-021-00386-6

8 - Marquevielle J, Robert C, Lagrabette O, Wahid M, Bourdoncle A, Xodo LE, Mergny JL, Salgado GF. 2020. Structure of two G-quadruplexes in equilibrium in the KRAS promoter. Nucleic Acids Res. https://doi.org/10.1093/nar/gkaa387

9 - Novel self-replicating α-synuclein polymorphs that escape ThT monitoring can spontaneously emerge and acutely spread in neurons.
De Giorgi F, Laferrière F, Zinghirino F, Faggiani E, Lends A, Bertoni M, Yu X, Grelard A, Morvan E, Habenstein B, Dutheil N, Doudnikoff E, Daniel J, Claverol S, Qin C, Latch A, Bezard E, Ichas F. Sci Adv. 2020 Oct 2;6(40):eabc4364. doi: 10.1126/sciadv.abc4364

10 - Tandem NMR and Mass Spectrometry Analysis of Human Nuclear Membrane Lipids. Dazzoni R, Buré C, Morvan E, Grelard A, Gounou C, Schmitter JM, Latch A, Larijani B, Dufourc EJ.
Anal Chem. 2020 May 19;92(10):6858-6868. doi: 10.1021/acs.analchem.9b05052

11 - The unprecedented membrane deformation of the human nuclear envelope, in a magnetic field, indicates formation of nuclear membrane invaginations. Dazzoni R, Grelard A, Morvan E, Bouter A, Applebee CJ, Latch A, Larijani B, Dufourc EJ. SciRep. 2020 Mar 20;10(1):5147. doi:10.1038/s41598-020-61746-0

12 - Structural dissection of amyloid aggregates of TDP-43 and its C-terminal fragments TDP-35 and TDP-16. Shenoy J, El Mammeri N, Dutour A, Berbon M, Saad A, Lends A, Morvan E, Grelard A, Lecomte S, Kauffmann B, Theillet FX, Habenstein B, Latch A. FEBS J. 2020 Jun;287(12):2449-2467. doi: 10.1111/feb.15159