BIP, CNRS/Aix-Marseille University
Laboratory of Bioenergetics and Protein Engineering (BIP, UMR 7281)
Mediterranean Institute of Microbiology (IMM, FR 3479)
31 Joseph Aiguier Road
13009 Marseille
FRANCE
Dr. Emilien Etienne (This e-mail address is protected against spambots. You need JavaScript enabled to view it.)
Electron Paramagnetic Resonance (EPR) is a central technique for the characterization of paramagnetic species. In the field of life sciences, it is particularly well suited to the molecular analysis of biological systems, their functions and their interactions. These paramagnetic species can be naturally present in biomolecules in the form of metal centers or free radicals. In this case, the EPR makes it possible to determine their nature and their magnetic properties, during a catalytic cycle for example. Paramagnetic probes, most often nitroxides, can also be inserted into biomolecules to study their dynamics and their interactions.
Although mainly dedicated to life science applications and developments at the chemistry-biology interface, the BIP platform is multidisciplinary. It benefits from the scientific support of 9 researchers and 2 engineers.
Equipment
Bruker ELEXSYS E580 Spectrometer
Bruker ELEXSYS E500 I Spectrometer
Bruker ELEXSYS E500 II Spectrometer
- Expertise and services
- oxidoreductases / metalloenzymes: metal content, analysis of metal cofactors, functioning of complex redox enzymes.
- Spin labeling coupled with EPR: dynamics of biomolecules (proteins, DNA, ...), interactions between macromolecules.
- Other equipment
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Bruker Magnettech MS 5000 benchtop spectrometer (X-band, room temperature),
- Glove box for sample preparation in anaerobiosis,
- Storage of samples in liquid nitrogen (77K) or in a freezer at -80°C.
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