In this research work, our team explored the possibility of using nuclear magnetic resonance (NMR) in cell, that is to say directly on bacterial cell cultures, to characterize the bacterial periplasm, a front-line cellular compartment. of the action of antibiotics. The objective was to follow the fate of these antibiotics in this compartment, in the presence or absence of the expression by the bacteria of β-lactamase enzymes linked to bacterial resistance. This study shows that in cell NMR constitutes a powerful analytical tool for the study of new molecules targeting the molecular components of the bacterial periplasm.
